Fig. 3.
Binding isotherm of 125I-crotalin on human platelet suspension. Platelets were incubated with various concentrations of 125I-crotalin. Total binding (▴) and nonspecific binding (•) in the presence of unlabeled crotalin (200 μg/mL) were determined, respectively. Specific binding (▪) was calculated by subtracting the nonspecific binding from total binding. This is a representative one of four similar experiments.