Tyrosine phosphorylation of RAFTK in CCR5-L1.2 cells and activated T cells in response to chemokines. (A) CCR5-L1.2 cells or (B) T cells were unstimulated (UN) or stimulated with 100 nmol/L or 250 nmol/L MIP1β for varying time intervals. Cells were lysed and immunoprecipitated with anti-RAFTK, and analyzed by immunoblotting with antiphosphotyrosine (anti-pTyr). The same immunoblot was stripped and immunoblotted with anti-RAFTK (lower panel).