Bar graph of transduction conditions of LAD CD34⁺ cells by retroviral vector PG13/LgCD18. Cells were transduced under the conditions shown and analyzed by FACS using FITC conjugated anti-human CD18. LgCD18, transduction using vector PG13/LgCD18. Mock, mock transduction using supernatant from PG13 cells only. Full Fib, full-length fibronectin; CH-296, recombinant fibronectin fragment CH-296; co-cult, cocultivation on producer line PG13/LgCD18. Data represent the average of two separate experiments. Error bars indicate the standard deviation. (▧), No protamine sulfate; (▪), 8 μg/mL protamine sulfate.