Flow cytometric analysis of untransduced LAD CD34⁺ cells, transduced LAD CD34⁺ cells, and normal CD34⁺ cells. LAD CD34⁺ cells were transduced using the retroviral vector PG13/LgCD18 in a 3-day supernatant transduction on CH-296. Untransduced LAD CD34⁺ cells and normal CD34⁺ cells were grown for 3 days in IMDM/30% plus growth factors. After 3 days, all three cell populations were immunostained with either anti-human CD18, CD11a, or CD11b (X-axis where noted) and CD34 (Y-axis). 7AAD was used to gate out dead cells (not shown).