Flow cytometric analysis of the cell surface expression of SCF isoforms on stromal cells. Stromal cells stably transfected with cDNAs encoding either the soluble (Sl/Sl4-S) or the MR (Sl/Sl4-MR) form of SCF were stained for flow cytometric analysis as described in Materials and Methods. (A) Parental Sl/Sl4 cells were stained with either isotype control (dotted line) or rat anti-mouse SCF (solid line) MoAb followed by FITC-conjugated goat F(ab′)2 anti-rat IgG secondary; (B) Sl/Sl4-S cells were stained with either isotype control (dotted line) or rat anti-mouse SCF (solid line) MoAb followed by FITC-conjugated goat F(ab′)2 anti-rat IgG secondary; (C) Sl/Sl4-MR cells were stained with either isotype control (dotted line) or rat anti-mouse SCF (solid line) MoAb followed by FITC-conjugated goat F(ab′)2 anti-rat IgG secondary.
