Fig. 5.
![Fig. 5. EMSA of 32Dcl3Neo+ and 32Dcl3CDP/cut nuclear proteins bound to the DM2 LF silencer oligomers following G-CSF induction. (A) Double-stranded DM2 oligomers were [γ-32P]dATP–labeled and incubated with equal concentrations (15 μg) of nuclear extracts prepared from uninduced (lane 2) and G-CSF–induced day 2 (lane 3) and day 4 (lane 4) 32Dcl3Neo+ cells. Lanes 1 and 5, labeled DM2 oligomers without addition of any nuclear extracts. (B) [γ-32P]dATP–labeled DM2 oligomers were incubated with 15 μg/lane of nuclear extracts prepared from uninduced (lane 6), G-CSF–induced (day 2, lane 7), and G-CSF–induced (day 4, lane 8) 32Dcl3CDP/cut cells. Arrows in A and B represent the CDP/cut protein-DNA complex.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/7/10.1182_blood.v90.7.2784/3/bl_0022f5.jpeg?Expires=1735833818&Signature=sBXRw5alInNbFLgduZatd97Bix1qGzkDsV7P4MDmhVU9hmLESP0mMX~Fq2Q2XljWRmUG05mf0xTHjpJ8MSATHiDc8vMrHuYWa6d6nFrFoseR3OkkA~8H69Ls-6NyEayq9fKXb4qHckiaRiML25Q6jwn9~GGPfA2zWMMbii~IXyy9RaLIrpLXhrfEOs5rqkzc2XG5lyKuzviocRM1~dTQoLZYUK6ufE6sjOhimhyLBx-lOMnu9gQPW0hEgeWH6CZFI4IKdd~8L26ixnZn468qCzyiIoe9F-enphKDav9Ud8478LRYCD8~M8ywtCnqmsBZfRItnNJREgCkG2AOJWbv8g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
EMSA of 32Dcl3Neo+ and 32Dcl3CDP/cut nuclear proteins bound to the DM2 LF silencer oligomers following G-CSF induction. (A) Double-stranded DM2 oligomers were [γ-32P]dATP–labeled and incubated with equal concentrations (15 μg) of nuclear extracts prepared from uninduced (lane 2) and G-CSF–induced day 2 (lane 3) and day 4 (lane 4) 32Dcl3Neo+ cells. Lanes 1 and 5, labeled DM2 oligomers without addition of any nuclear extracts. (B) [γ-32P]dATP–labeled DM2 oligomers were incubated with 15 μg/lane of nuclear extracts prepared from uninduced (lane 6), G-CSF–induced (day 2, lane 7), and G-CSF–induced (day 4, lane 8) 32Dcl3CDP/cut cells. Arrows in A and B represent the CDP/cut protein-DNA complex.
