Characterization of LEC-1 clones. (A) Phase contrast microscopy of attached LEC-1 (A12 clone) 2 days after culture in IMDM 20% FCS. Note the spindle like morphology of these endothelial cells (original magnification × 20). Fluorescence-activated cell sorting (FACS) analysis of LEC-1 showing the expression of CD34 (B), VCAM-1 (C), and ICAM-1 (D) (thin line). Negative controls were stained with the respective isotype (black profile). Similar results were obtained with the C7 clone.