Representative Southern Blot analysis of a nested PCR by using KS1 and KS2 primers to amplify HHV-8 DNA sequences. (A) Lane 1, positive control; lanes 2 to 4 and 6 to 7, lesional skin from C-KS and AIDS-KS patients, respectively; lane 5, uninvolved skin; lane 8, negative control. (B) Lesional skin cultures from C-KS patients: lane 1, positive control; lane 2, negative control; lanes 3 and 4, cultured skin from 2 C-KS patients. (C) PBMC: lane 1, positive control; lane 2, negative control; lanes 3 to 6, PBMC from four C-KS patients; lane 4, PBMC from an AIDS-KS patient.