(A) Strategy for the AS-PCR. Each allele is distinguished by its product size. A common product is amplified from both alleles by the primers F1 and R1. The primer Fm recognizes only the mutant allele sequence while the primer Rw recognizes only the wild-type sequence. *G to A mutation (C282Y) creates a new Rsa I site. (B) Results of 20 subjects screening demonstrating 16 normal subjects, 3 carriers (*) and 1 homozygote (†). C, common product; M, mutant allele; W, wild-type allele; Mk, size marker (Alu I–digested pUC19).