Comparison of ploidy distribution of MKs and the expression of CD41b in different ploidy classes in the MKs obtained in the presence of PEG-rHuMGDF or the combination of IL-3, SCF, and IL-6 from CD34⁺ CD41⁺ cells. Marrow CD34⁺ CD41⁺ cells were grown from 6 days in serum-free conditions, in the presence of PEG-rHuMGDF (10 ng/mL) or IL-3 (100 U/mL) plus SCF (50 ng/mL) plus IL-6 (100 U/mL). Ploidy of the CD41⁺ cells obtained in the presence of PEG-rHuMGDF (A) and IL-3 plus SCF plus IL-6 (B). Comparison of the expression of CD41b in MK cultivated in the presence of PEG-rHuMGDF (solid line) and the combination of IL-3, SCF and IL-6 (broken lines) in the 2N (C), 4N (D), 8N (E), 16N (F), and 32N MKs (G). MKs were considered as CD41b⁺ cells and analysis was performed in each ploidy class.