(A) Adhesion of CD34 cells isolated from mini-Macs to BSA and H0 and H120 fragments coated at different concentrations. All adhesion experiments were for 30 minutes at 37°C. All histograms in all figures for adhesion and migration represent the mean of duplicate wells ± SD. Student's t-test was used to calculate P values. (B) Adhesion of CD34 cells to H120 fragment coated at 15 μg/mL showing inhibition by blocking anti-α4 and anti-β1 antibodies, but not by IgG control. (C) Adhesion of CD34 cells to BSA and H0 and H120 fragments coated at 15 μg/mL with and without a mixture of growth factors; IL-3 (10 ng/mL), SCF (100 ng/mL), G-CSF (500 ng/mL), and IL-6 (200 U/mL). Reduction in adhesion to H120 induced by growth factors was significant (P < .05). (D) Adhesion of CD34 cells to H120 fragment coated at 15 μg/mL with IL-3 10 ng/mL, SCF 100 ng/mL, and G-CSF 500 ng/mL added together and separately at different concentrations. Changes in adhesion were significant for all growth factors (P < .005), IL-3 10 ng/mL (P < .01), IL-3 100 ng/mL (P < .005), and SCF (P < .05).
