Stable ectopic expression of WT1 +KTS isoforms, but not −KTS isoforms, in M1 cells. (A) Northern blotting of total RNA followed by hybridization with probes to WT1 and β-actin, with the 28S rRNA band shown below to illustrate equal loading of the samples on the gel, and the position of the 28S and 18S rRNA bands indicated to the left of the autoradiographs. (B) Western blotting of protein lysates followed by hybridization with an antibody specific for WT1, with an identical gel stained for total protein shown below to illustrate equal loading of the samples, and the size and position of the protein molecular weight markers indicated to the left of the autoradiograph.