Expression of c-fms and Mac-1 cell surface myelomonocytic markers in M1.WT1 +KTS cells. (A) Northern blotting of total RNA followed by hybridization with probes to c-fms and β-actin, with the 28S rRNA band shown below to illustrate equal loading of the samples on the gel, and the position of the 28S and 18S rRNA bands indicated to the left of the autoradiographs. (B) Flow cytometric analysis using a biotinylated antibody specific for Mac-1 followed by fluorescent detection with a streptavidin-FITC secondary conjugate, with control cells stained with secondary conjugate alone. (- - -), Control; (—), Mac-1.