Phenotypic analysis of DC preparations used in this study. Purified thymic and splenic DC from either BALB.D2 or BALB/c mice were tripled stained with PE-conjugated anti-MHC II, FITC-conjugated anti-B220 and anti-CD3, and biotin-conjugated anti-CD11c followed by streptavidin-tricolor. The figure shows the MHC II versus B220 + CD3 contour profile of BALB.D2 thymic and splenic DC, as well as the corresponding histograms for CD11c and FSC (black profiles). White profiles in the CD11c histograms represent streptavidin-tricolor background staining of the DC preparation. White profiles in the FSC histograms of the thymic and splenic DC preparations represent the FSC of total thymocytes and total splenocytes, respectively. The results shown demonstrate that the DC preparations had a purity greater than 90%. Similar results were obtained for DC isolated from BALB/c mice.