Fig. 4.
Surface prion protein expression by HL-60 cells persists upon induction of monocyte lineage differentiation. HL-60 cells were cultured in the presence or absence of 100 nmol/L PMA for 3 days, then stained for flow cytometry analysis with MoAb 3F4. Histograms show PrPC surface immunoreactivity relative to an IgG2b isotype control. Minimal change is observed in PrPC staining intensity between undifferentiated (A) and differentiated cells (B).