Fig. 5.
Fig. 5. Association of AML1/ETO(MTG8) and AML1/Evi-1 with PEBP2β. COS-7 cells (1 × 106) were transfected with 10 μg of the expression plasmids for full-length or mutant chimeric protein together with 1 μg of that for PEBP2β. Cells were lysed, immunoprecipitated with anti-AML1 serum, and subjected to Western blotting with anti-AML1 serum or rabbit anti-PEBP2β serum. Arrowheads show the positions of full-size and mutant chimeric proteins. The position of PEBP2β is marked by the arrow. Molecular weight standards (in kilodaltons) are indicated.

Association of AML1/ETO(MTG8) and AML1/Evi-1 with PEBP2β. COS-7 cells (1 × 106) were transfected with 10 μg of the expression plasmids for full-length or mutant chimeric protein together with 1 μg of that for PEBP2β. Cells were lysed, immunoprecipitated with anti-AML1 serum, and subjected to Western blotting with anti-AML1 serum or rabbit anti-PEBP2β serum. Arrowheads show the positions of full-size and mutant chimeric proteins. The position of PEBP2β is marked by the arrow. Molecular weight standards (in kilodaltons) are indicated.

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