Fig. 4.
Fig. 4. Western analysis of tyrosine phosphorylation of TrkA in HMC-1 mast cells stimulated with NGF. HMC-1 cells were stimulated with NGF (200 ng/mL) for 5 or 10 minutes. TrkA was immunoprecipitated with specific anti-TrkA antibody from lysates of NGF-treated (+) or untreated (−) cells, and the immunoprecipitates were probed with the RC20 antiphosphotyrosine antibody.

Western analysis of tyrosine phosphorylation of TrkA in HMC-1 mast cells stimulated with NGF. HMC-1 cells were stimulated with NGF (200 ng/mL) for 5 or 10 minutes. TrkA was immunoprecipitated with specific anti-TrkA antibody from lysates of NGF-treated (+) or untreated (−) cells, and the immunoprecipitates were probed with the RC20 antiphosphotyrosine antibody.

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