Expression of mRNA transcripts encoding trkA, trkB, and trkC in HMC-1 cells, human cord blood-derived mast cells, and mast cells purified from human lungs. The cDNA was synthesized from total RNA (HMC-1) or heparinase-treated total RNA (human umbilical cord blood-derived or lung mast cells) by reverse transcriptase and amplified by PCR using degenerate oligonucleotide primers A and B. The identity of the PCR products was confirmed by Southern blot analysis using 32P-labeled human trkA, monkey trkB, and monkey trkC cDNA probes. (+) denotes specimens from cells that were sensitized with human IgE and subsequently stimulated with antihuman IgE, whereas (−) denotes specimens from cells not stimulated with antihuman IgE (see Materials and Methods for details).
