American Society of Hematology

Fig. 4.

$Fig. 4. Two-dimensional electrophoresis of PI3K immune complexes phosphorylated, in vitro. PI3K was immunoprecipitated from, post-130,000g, cytosolic fractions of resting platelet lysates and the immune complexes subjected to an in vitro protein kinase assay in the presence of Mn2+ and γ-32P-ATP in the absence (CO) or presence of 30 μmol/L LY294002. Radiolabeled proteins were eluted with urea sample buffer and analyzed by two-dimensional electrophoresis as described in Materials and Methods. Spots 1, 2, and 3 are unidentified reference points used for relative quantitation (see Results). Molecular mass markers in kilodaltons are indicated at the left. IEF; isoelectric focusing.$

Two-dimensional electrophoresis of PI3K immune complexes phosphorylated, in vitro. PI3K was immunoprecipitated from, post-130,000g, cytosolic fractions of resting platelet lysates and the immune complexes subjected to an in vitro protein kinase assay in the presence of Mn²⁺ and γ-³²P-ATP in the absence (CO) or presence of 30 μmol/L LY294002. Radiolabeled proteins were eluted with urea sample buffer and analyzed by two-dimensional electrophoresis as described in Materials and Methods. Spots 1, 2, and 3 are unidentified reference points used for relative quantitation (see Results). Molecular mass markers in kilodaltons are indicated at the left. IEF; isoelectric focusing.

This Feature Is Available To Subscribers Only