KSHV cyclin expression in BC-3 and BJAB cell suspension mixtures. (A) 80% BC-3 (KSHV-positive), 20% BJAB (KSHV-negative); (B) 20% BC-3, 80% BJAB. These results show appropriate percentages of cells with punctate labeling indicating that diffusion of the label between cells, a possible artifact of PCR-ISH-based assays, does not occur in this procedure.