Fig. 2.
![Fig. 2. Representative results of E2A-PBX1 PCR assays. Photograph of an ethidium bromide-stained agarose gel containing size-fractionated products of nested PCR forE2A-PBX1 fusion mRNA. Molecular size (MW) markers are indicated at left in base pairs. The sample identity is given at the top of the gel. Samples include bone marrow (B) and/or peripheral blood (P) from weeks 25 and/or 31 for patients 1 to 6, t(1;19)+ RCH-ACV and t(1;19)− REH pre-B ALL cell lines, a 10−4 dilution of RCH-ACV RNA into REH RNA, and RT and PCR negative controls. The migration of the E2A-PBX1PCR product is indicated at the right of the gel. For patient 2, two independently isolated BM samples [Ba and Bb] were analyzed.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/3/10.1182_blood.v91.3.1021/3/blod4032202.jpeg?Expires=1735555098&Signature=Krj5uOxHhyGMW-1LqdKGNO~etroMhutPL~j5cYImEfQFhPo8eEhtmNIRJJBKtrSa-p9cPasNDUrc-lz1ZoOqfgVzeqAZ4UNUEYsoLbK1b8QnY8CRVYvzr5eDOPHQper1qOwqvXXzJvboSpsavxBvPi0CBj58Z9zDlPL0bP96UYHsm9Wbcp71qyPoqv1v8u2A5KYM6sR9kWzA9-oVZmftwr5AxMfvYSqqG-JpaVs~chOs0xCR~VarK-azWLqYNGFzh-RSAevWs0b5VxRJRrqUlZCc9HpLlNdyLa1plgrWdXVlqIZ9PlOJbvKhc4uB3NynXm73h705XJYFKybKf-FUUA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Representative results of E2A-PBX1 PCR assays. Photograph of an ethidium bromide-stained agarose gel containing size-fractionated products of nested PCR forE2A-PBX1 fusion mRNA. Molecular size (MW) markers are indicated at left in base pairs. The sample identity is given at the top of the gel. Samples include bone marrow (B) and/or peripheral blood (P) from weeks 25 and/or 31 for patients 1 to 6, t(1;19)+ RCH-ACV and t(1;19)− REH pre-B ALL cell lines, a 10−4 dilution of RCH-ACV RNA into REH RNA, and RT and PCR negative controls. The migration of the E2A-PBX1PCR product is indicated at the right of the gel. For patient 2, two independently isolated BM samples [Ba and Bb] were analyzed.
