Expression of ATM in T-PLLs . Detergent lysates (60 μg/lane) were subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) through a 5% gel. Immunoblot analysis was performed using the anti-ATM antiserum pAB 132 (upper panel). Ponceau S staining was used to verify equal loading and protein integrity (lower panel). Samples analyzed were T-PLLs with LOH of the 11q22-23 region: Dia, Big, Bul, Imb, and Lec; T-PLLs without LOH: Ver and Ber; normal peripheral lymphocytes (PBL); normal thymocytes (Thy). Molecular weight standards are given in kD. The ATM specific signal is arrowed.