Western analysis of cytosolic levels of cyt c (A); 116-kD³⁵S-labeled, in vitro–translated PARP or its cleaved products (B); 45-kD DFF subunit or its 11-kD cleavage fragment (C); as well as the internucleosomal DNA fragmentation in the untreated and HIDAC-treated HL-60/neo (D, lanes 1 and 2), HL-60/Bcr-Abl (lanes 3 and 4), or K562 cells (lanes 5 and 6). Lane M in (D) represents the 123-bp marker DNA ladder. (E and F) Show Western analyses of the levels of cytosolic cyt c (E) and PARP or its cleaved products (F) in the untreated or etoposide-treated HL-60/neo or HL-60/Bcr-Abl cells.