American Society of Hematology

Fig. 4.

$Fig. 4. Anti–Stat-3 immunoblot of the cytoplasmic and nuclear fraction of Jurkat 76.25 cells after MHC-I ligation. Cells (3 × 107) were preincubated with PBS (lane 1) or saturating amounts of anti-β2M Ab (lanes 2 and 3) before exposure to avidin for the indicated time. Cells were separated in a cytoplasmic (A) or nuclear (B) fraction as described in the Materials and Methods. The separated fractions were immunoblotted with anti–Stat-3 Ab.$

Anti–Stat-3 immunoblot of the cytoplasmic and nuclear fraction of Jurkat 76.25 cells after MHC-I ligation. Cells (3 × 10⁷) were preincubated with PBS (lane 1) or saturating amounts of anti-β₂M Ab (lanes 2 and 3) before exposure to avidin for the indicated time. Cells were separated in a cytoplasmic (A) or nuclear (B) fraction as described in the Materials and Methods. The separated fractions were immunoblotted with anti–Stat-3 Ab.

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