American Society of Hematology

Fig. 2.

$Fig. 2. Downregulation of both IgE and FcRIα during culture in the absence of IgE in the culture medium (n = 4). Both parameters were measured by flow cytometry, IgE with TES-19 anti-IgE monoclonal antibody (•) and FcRIα (○) with monoclonal antibody 22E7. On average, the starting levels of expression for FcRIα were 110 ± 25 flow fluorescence units, equivalent to ≈130,000 FcRIα per cell (see Materials and Methods). Data are expressed as a fraction of the day 0 level of expression. (B and C) Representative flow-cytometric profiles for 1 of the experiments; (B) day 0 distribution (mean fluorescence, 63), and (C) day 17 distribution (mean = 17) using 22E7 to detect FcRIα.$

Downregulation of both IgE and FcRIα during culture in the absence of IgE in the culture medium (n = 4). Both parameters were measured by flow cytometry, IgE with TES-19 anti-IgE monoclonal antibody (•) and FcRIα (○) with monoclonal antibody 22E7. On average, the starting levels of expression for FcRIα were 110 ± 25 flow fluorescence units, equivalent to ≈130,000 FcRIα per cell (see Materials and Methods). Data are expressed as a fraction of the day 0 level of expression. (B and C) Representative flow-cytometric profiles for 1 of the experiments; (B) day 0 distribution (mean fluorescence, 63), and (C) day 17 distribution (mean = 17) using 22E7 to detect FcRIα.

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