Changes in the cell-surface expression of FcRIα as measured by 22E7 binding or changes in total cell FcRIα as measured by Western blotting. (A) An example of the Western blot data for cells at day 0 or day 21 after culture. The number of total cells is held constant and the basophil purity was found to be the same for the 2 time points, averaging 41% ± 13% for the 5 experiments. (B) Average data for flow-cytometric measurements or Western blotting in terms of day 0 levels (n = 5) Western blot films were digitally imaged to determine optical densities of the bands. The difference in relative change as measured by flow cytometry or Western blotting was not statistically significant, while both were significantly less than day 0 (P < .001).