American Society of Hematology

Fig. 5.

$Fig. 5. Upregulation of FcRIα of different types of basophil preparations under the influence of 500 ng/mL of PS myeloma IgE during a 7-day culture. The four symbols represent different methods to obtain basophils; (*) basophils obtained from leukapheresis packs and incubated for 21 days before addition of IgE, (○) basophils obtained from leukapheresis packs but cultured for 7 days without the downregulation step of 21-day culture, (•) basophils obtained from standard venipuncture and double Percoll gradient separation and incubated for 21 days before addition of IgE, (▪) basophils obtained from standard venipuncture and double Percoll gradient separation but cultured for 7 days without the downregulation step of a 21-day culture. Each symbol represents the results from a single donor. The starting receptor density (x-axis) is the 22E7 fluorescence by flow cytometry and the fractional upregulation (y-axis) is the ratio of day 7 fluorescence to day 0 fluorescence.$

Upregulation of FcRIα of different types of basophil preparations under the influence of 500 ng/mL of PS myeloma IgE during a 7-day culture. The four symbols represent different methods to obtain basophils; (*) basophils obtained from leukapheresis packs and incubated for 21 days before addition of IgE, (○) basophils obtained from leukapheresis packs but cultured for 7 days without the downregulation step of 21-day culture, (•) basophils obtained from standard venipuncture and double Percoll gradient separation and incubated for 21 days before addition of IgE, (▪) basophils obtained from standard venipuncture and double Percoll gradient separation but cultured for 7 days without the downregulation step of a 21-day culture. Each symbol represents the results from a single donor. The starting receptor density (x-axis) is the 22E7 fluorescence by flow cytometry and the fractional upregulation (y-axis) is the ratio of day 7 fluorescence to day 0 fluorescence.

This Feature Is Available To Subscribers Only