Western blot analysis of solubilized extracellular vesicles harvested from MIP-101 conditioned medium. Vesicles extracted with n-octyl β-D-glucopyranoside and treated with N-glyconase before SDS-PAGE produce a single band with an apparent molecular weight of 45 kD when probed with anti-Fas antibody (N-gly). Vesicles extracted with detergent and subjected to SDS-PAGE without N-glyconase treatment show two bands with apparent molecular weights of 45 and 48 kD when probed with anti-Fas antibody (C).