Fig. 7.
Synthesis of Fas by CX-1 and MIP-101 cells. CX-1 (□) and MIP-101 (▧) cells were cultured in methionine-free medium for 6 or 16 hours with 300 μCi 35S-methionine. Cells were lysed with Triton X-100 and the lysates were immunoprecipitated sequentially with goat IgG and goat anti-Fas IgG antibody. Radioactivity in aliquots (one fifth of the total sample) of each immunoprecipitated sample was determined by scintillation. Note that Fas synthesis is greater for MIP-101 cells than for CX-1 cells (P < .05). Shown are mean values of a single experiment. Similar results were obtained in two additional studies.
