Effect of FasL-bearing extracellular vesicles on CX-1 cell viability. Negative and positive controls were established by incubating 2 × 105 CX-1 cells with 40 μg total membrane protein prepared from vesicles shed from CX-1 cells (CX-1) or 100 ng antihuman Fas IgM (Anti-Fas), respectively. Vesicles shed from Hut 78 cells suppress viability by nearly 40% (Hut 78 Alone). Whereas the addition of 40 μg total membrane protein of vesicles shed from MIP-101 cells restores nearly full viability (Hut 78 + MIP-101), the addition of 40 μg total membrane protein of vesicles shed from CX-1 cells has a minimal effect (Hut 78 + CX-1). Note that the viability of cells incubated with mixtures of vesicles prepared from Hut 78 cells and MIP-101 cells is increased, relative to that of cells incubated with mixtures of vesicles prepared from Hut 78 cells and CX-1 cells (P < .01). Shown are the mean ± SD values in three separate experiments.
