Fig. 6.
![Fig. 6. Simultaneous determination of apoptosis (ISEL) and DNA content (Hoechst 33324) in B-CLL CD19+ lymphocytes after 24-hour treatment in vitro with 3 μmol/L fludarabine (patient No. 5, group 1). B-CLL cells are mainly in the G0/1 cell cycle phases when they become positive for ISEL. Among the CD19+/ISEL+ lymphocytes, cells with diploid DNA content (early apoptotic cells, region [reg] 1) can be distinguished from cells with subdiploid DNA content (late apoptotic cells, reg 2).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/5/10.1182_blood.v91.5.1742/3/blod4052306.jpeg?Expires=1735500684&Signature=lOIrWMF4kr5iQItDeVVPFXk9IGT7pQaZGE6vg2uBuhWgO1llMND6q66jn7Fcm37eLjPXe50uXpI059~ABePyo4oak2iq8xlCYZVwASYoi8uCzIypXTz6cqgxMAlodgf7b1Im4ks~57tRfoZO1uoDX9wMqMBdbOjUBGgq1wLVpUVJi1UKh2eADo1CQzECGVFk88b9hDbbt51yfM8Tbi89aZm~T63RKl7IVQ2Pj5R~KCtftja-qubGmL1i7otk8pbF74SLRz0VrqFQehyjH~4bAJJNOS3GyYq9f6ORWUZmx6bWU55RLFW9Jq1nUGGgHBNII4L6VBPQOgUWdMt-DbngbA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Simultaneous determination of apoptosis (ISEL) and DNA content (Hoechst 33324) in B-CLL CD19+ lymphocytes after 24-hour treatment in vitro with 3 μmol/L fludarabine (patient No. 5, group 1). B-CLL cells are mainly in the G0/1 cell cycle phases when they become positive for ISEL. Among the CD19+/ISEL+ lymphocytes, cells with diploid DNA content (early apoptotic cells, region [reg] 1) can be distinguished from cells with subdiploid DNA content (late apoptotic cells, reg 2).
