American Society of Hematology

Fig. 1.

$Fig. 1. Stimulation of PKC translocation by TPO in UT-7/mpl 5.1 cells. (A) Effect of TPO or PMA on the subcellular distribution of total PKC activity. Quiescent cells were treated for 15 minutes with 40 ng/mL TPO or 50 nmol/L PMA and then PKC activity was measured in the membrane and cytosolic fractions. Values represent the mean ± SD of four independent experiments. (□), control; (▪), TPO; (▨), PMA. *P < .05, **P < .01. (B) Time course of TPO-induced increase in membrane-associated PKC activity. Cells were treated with 40 ng/mL TPO for the indicated times and then PKC activity was measured in the membrane fraction. (C) Dose response for the increase in membrane-associated PKC activity induced by TPO. Cells were treated with the indicated concentrations of TPO for 15 minutes and then PKC activity was measured in the membrane fraction. PKC activity is expressed relative to the level present in unstimulated cells incubated in parallel. All other experimental details were as described in Materials and Methods.$

Stimulation of PKC translocation by TPO in UT-7/mpl 5.1 cells. (A) Effect of TPO or PMA on the subcellular distribution of total PKC activity. Quiescent cells were treated for 15 minutes with 40 ng/mL TPO or 50 nmol/L PMA and then PKC activity was measured in the membrane and cytosolic fractions. Values represent the mean ± SD of four independent experiments. (□), control; (▪), TPO; (▨), PMA. *P < .05, **P < .01. (B) Time course of TPO-induced increase in membrane-associated PKC activity. Cells were treated with 40 ng/mL TPO for the indicated times and then PKC activity was measured in the membrane fraction. (C) Dose response for the increase in membrane-associated PKC activity induced by TPO. Cells were treated with the indicated concentrations of TPO for 15 minutes and then PKC activity was measured in the membrane fraction. PKC activity is expressed relative to the level present in unstimulated cells incubated in parallel. All other experimental details were as described in Materials and Methods.

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