American Society of Hematology

Fig. 2.

$Fig. 2. Effect of TPO on the subcellular localization of PKC-α and -β isoforms as detected by immunoblotting. Quiescent UT-7/mpl 5.1 cells were treated for 15 minutes with 40 ng/mL TPO, 2 ng/mL GM-CSF or 50 nmol/L PMA as indicated. Cytosolic and membrane fractions were prepared from each sample and aliquots equivalent to 5 × 105 cells in case of the membrane fraction, or half that amount in case of the cytosolic fraction, were analyzed by immunoblotting using MoAbs against the indicated PKC isoforms, as described in Materials and Methods.$

Effect of TPO on the subcellular localization of PKC-α and -β isoforms as detected by immunoblotting. Quiescent UT-7/mpl 5.1 cells were treated for 15 minutes with 40 ng/mL TPO, 2 ng/mL GM-CSF or 50 nmol/L PMA as indicated. Cytosolic and membrane fractions were prepared from each sample and aliquots equivalent to 5 × 10⁵ cells in case of the membrane fraction, or half that amount in case of the cytosolic fraction, were analyzed by immunoblotting using MoAbs against the indicated PKC isoforms, as described in Materials and Methods.

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