American Society of Hematology

Fig. 3.

$Fig. 3. Effect of GF109203X on the membrane-associated PKC activity translocated by TPO. (A) Inhibition of PMA-dependent or TPO-dependent membrane-associated PKC activity by GF109203X in intact cells. Quiescent UT-7/mpl 5.1 cells were preincubated for 15 minutes in the absence (□) or presence (▪) of 3 μmol/L GF109203X, and then treated for another 15 minutes with 40 ng/mL TPO or 50 nmol/L PMA. At the end of this period PKC activity was measured in the membrane fraction as described in Materials and Methods. Results represent the mean of four independent experiments ± SD. PKC activity is expressed relative to the level present in unstimulated cells incubated in parallel. (B) Inhibition of membrane-associated PKC activity as a function of GF109203X concentration in the assay. Cells were treated for 15 minutes with 40 ng/mL TPO and then PKC activity was measured in the membrane fraction in the presence of the indicated concentrations of GF109203X. Results are expressed as the percentage of PKC activity in the sample with no GF109203X added.$

Effect of GF109203X on the membrane-associated PKC activity translocated by TPO. (A) Inhibition of PMA-dependent or TPO-dependent membrane-associated PKC activity by GF109203X in intact cells. Quiescent UT-7/mpl 5.1 cells were preincubated for 15 minutes in the absence (□) or presence (▪) of 3 μmol/L GF109203X, and then treated for another 15 minutes with 40 ng/mL TPO or 50 nmol/L PMA. At the end of this period PKC activity was measured in the membrane fraction as described in Materials and Methods. Results represent the mean of four independent experiments ± SD. PKC activity is expressed relative to the level present in unstimulated cells incubated in parallel. (B) Inhibition of membrane-associated PKC activity as a function of GF109203X concentration in the assay. Cells were treated for 15 minutes with 40 ng/mL TPO and then PKC activity was measured in the membrane fraction in the presence of the indicated concentrations of GF109203X. Results are expressed as the percentage of PKC activity in the sample with no GF109203X added.

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