Southern blot (A) and PCR (B) for KSHV in various mouse tissues. (Note Southern blots were done independently of PCR and do not represent blotting of PCR products.) MW, molecular markers. Lane 1: Southern blot and PCR-positive ascitic cells from HBL-6 ip-injected mouse. Lane 2: Southern blot and PCR-positive tumor cells from HBL-6 iv-injected mouse. Lane 3: PCR-positive small intestine from HBL-6 iv-injected mouse. Lane 4: Southern blot and PCR-positive testis from HBL-6 iv-injected mouse. Lane 5: Southern blot and PCR-positive kidney from HBL-6 iv-injected mouse. Lane 6: PCR-positive heart from HBL-6 iv-injected mouse. Lane 7: Southern blot and PCR-positive lungs from HBL-6 iv-injected mouse. Lane 8: PCR-positive spleen from HBL-6 iv-injected mouse. Lane 9: Blank. Lane 10: PCR-positive kidney from BCP-1 iv-injected mouse. Lane 11: PCR-positive (weakly) lung from BCP-1 iv-injected mouse. Lane 12: PCR-positive (weakly) liver from BCP-1 iv-injected mouse. Lane 13: PCR-positive thymus from BCP-1 iv-injected mouse.