Pedigree Z-34. Filled symbols designate iron overload defined from liver biopsy (individual no. 1), high transferrin saturation and ferritin (individuals no. 8, 9, and 11), a probability >90% of heterozygosity in the analyses of transferrin saturation and unsaturated iron binding capacity (individual no. 4), or a probability >70% in the analyses of ferritin and the ferritin to AST ratio (individuals no. 3, 4, and 7). Open symbols designate studied individuals for whom iron overload criteria were not met. Shaded symbols designate unstudied individuals. Individual no. 5 was assigned unaffected status in the analysis because of low transferrin saturation and ferritin to AST ratio despite consuming >1,000 L beer. Individual no. 6 had a probability >90% of being a normal homozygote in the analyses of transferrin saturation and unsaturated iron binding capacity.