American Society of Hematology

Fig. 5.

$Fig. 5. Tec can constitutively associate with Jak2 in Sf21 cells. Sf21 cells were infected with baculovirus expressing Tec (T), Jak2 (J), TecKM (TM), and Jak2KE(JE) in the combinations indicated at the top. Jak2 was immunoprecipitated from each cells lysed by the 0.1%-lysis buffer (αJak IP), and probed with either anti-Tec serum (αTec) or anti-Jak2 serum (αJak2). Total cell lysates (TCL: 10 μg/lane) of each fraction were also probed with anti-Tec serum to estimate the expression level of Tec.$

Tec can constitutively associate with Jak2 in Sf21 cells. Sf21 cells were infected with baculovirus expressing Tec (T), Jak2 (J), Tec^KM (T^M), and Jak2^KE(J^E) in the combinations indicated at the top. Jak2 was immunoprecipitated from each cells lysed by the 0.1%-lysis buffer (αJak IP), and probed with either anti-Tec serum (αTec) or anti-Jak2 serum (αJak2). Total cell lysates (TCL: 10 μg/lane) of each fraction were also probed with anti-Tec serum to estimate the expression level of Tec.

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