Fig. 1.
Fig. 1. Expression of Bcl-2, Mcl-1 Bcl-xL and Bax in human leukemia cell lines. Aliquots containing 3 × 105cells from the leukemia cell line (lanes 1 through 9) were subjected to SDS-PAGE followed by blotting with antisera that recognize Bcl-2 (A), Bcl-xL (B), Mcl-1 (C), or Bax (D). As a control for loading and transfer of these samples, the same blots were probed with antibodies to histone H1 (E), a polypeptide that is present in constant amounts in all diploid cells. To provide a basis for comparison of polypeptide levels, serial dilutions of HL-60 cells or CEM cells transfected with Bcl-2 were loaded on the same blots. These samples contained 1.5 × 105, 0.75 × 105, 0.3 × 105, or 0.15 × 105 cells (lanes a through d, respectively).

Expression of Bcl-2, Mcl-1 Bcl-xL and Bax in human leukemia cell lines. Aliquots containing 3 × 105cells from the leukemia cell line (lanes 1 through 9) were subjected to SDS-PAGE followed by blotting with antisera that recognize Bcl-2 (A), Bcl-xL (B), Mcl-1 (C), or Bax (D). As a control for loading and transfer of these samples, the same blots were probed with antibodies to histone H1 (E), a polypeptide that is present in constant amounts in all diploid cells. To provide a basis for comparison of polypeptide levels, serial dilutions of HL-60 cells or CEM cells transfected with Bcl-2 were loaded on the same blots. These samples contained 1.5 × 105, 0.75 × 105, 0.3 × 105, or 0.15 × 105 cells (lanes a through d, respectively).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal