p32/p30 phosphorylation in D/V and D/C cells was mainly induced at 2 to 18 hours after Epo stimulation. Growth factor-deprived D/V and D/C cells were stimulated with Epo for various times. Cell lysates were prepared and used for immunoprecipitation with an anti–SHP-1 antibody. The cell lysates and immunocomplexes were analyzed by SDS-PAGE/Western blotting with antibodies as indicated. The positions of phosphoproteins and protein size markers are indicated.