Flow cytometric analysis of CD34⁺, CD34⁺CD38⁺, and CD34⁺CD38⁻ cell subsets used to initiate BMLTC. (A) Scatter diagram of CD34⁺ selected cells stained with FITC-labeled CD34 MoAb and PE-labeled CD38 MoAb, in which Log PE fluorescence intensity was displayed versus Log FITC fluorescence intensity. (B) CD34⁺ cells were gated and sorted. (C) A scatter diagram of gated CD34⁺ cells sorted for absence of the CD38 activation marker (most-primitive BM stem cells). (D) The log PE fluorescence intensity (CD38) of an affinity column purified CD34⁺ cell population was displayed versus Log PerCP-fluorescence intensity (CD4). Results of one representative experiment illustrated.