Fig. 3.
Effect of MoAb 724 to vWF on SIPA. Washed platelet suspensions (1.5 × 108/mL) and either buffer (▧) or purified vWF (10 μg/mL, ▪) were exposed to a shear rate of 4,000 seconds−1 for 5 minutes at 20°C in the presence of IgG of MoAb 418 as control, IgG of MoAb 724, or F(ab′)2 fragments of MoAb 724 (20 μg/mL). DSP was calculated as outlined in the legend to Fig 1. Means ± SEM were calculated from three experiments performed in duplicate. In the presence of MoAb 724 or its F(ab′)2 fragments, a significant enhancement of SIPA was observed in the vWF-containing samples, whereas this effect was not seen in the vWF-free samples. *P < .05, for the effect of MoAb 724 (IgG or F(ab′)2 fragments) versus MoAb 418 in the vWF-containing samples.
