Fig. 5.
![Fig. 5. Kinetics of [3H]-lyso-PI uptake. Transfer of [3H]-lyso-PI from donor vesicles to the cell membrane of lymphocytes was measured by scintillation counting of the incubation medium from EGTA-containing samples. The 100% value was obtained by measuring an aliquot of solution of 11.25 μmol/L [3H]-lyso-PI (concentration of substrate added to the cells). The kinetics of spontaneous uptake of the lipid by the cells shows that its concentrations in the medium plateaued at about 25% within 5 minutes. Beyond this time, the rate of lyso-PI transfer was evidently reduced. Data are the mean ± SD of at least 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/10/10.1182_blood.v91.10.3833/3/blod41041005y.jpeg?Expires=1735545386&Signature=tXNBMWx9zW224FzofQXPIhZ9zvpRkdogOI6tpc336L~AF5L0rAJRLF6~qC7I~1NFHkO8S7Ga1f0CjvN9S1mEnwk36zay1F3AHNc8b9kLS4KiH5-vlE-MowT~oVKhiSflybZlRa7NthKWq6HbEMLKnPFt-UvMCcG3c0pZnTp-BVNFSn2RVUUeikFOYyb8ix5TJJk0zoAhy5PLSHsL2A4Rv3nApP3GpebdkGu5t~XGOyglNYkJi5hAepWyahTlUz6XnLVdxKkjbc87b3YKcK5P5nfzIpZuYF5gIThscTq~jFxrq5m2W~buHqGcYK9tDtLRG~RZex248wHWqsxrR8VVhQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Kinetics of [3H]-lyso-PI uptake. Transfer of [3H]-lyso-PI from donor vesicles to the cell membrane of lymphocytes was measured by scintillation counting of the incubation medium from EGTA-containing samples. The 100% value was obtained by measuring an aliquot of solution of 11.25 μmol/L [3H]-lyso-PI (concentration of substrate added to the cells). The kinetics of spontaneous uptake of the lipid by the cells shows that its concentrations in the medium plateaued at about 25% within 5 minutes. Beyond this time, the rate of lyso-PI transfer was evidently reduced. Data are the mean ± SD of at least 3 separate experiments.
