American Society of Hematology

Fig. 7.

$Fig. 7. Percentage of radiolabeled inositol phosphates in the water-soluble material (100%). Lymphocytes in the different experimental conditions were incubated in a reaction mixture containing 11.25 μmol/L lyso-PI up to 30 minutes. Once the reaction was stopped, the water-soluble products extracted from the incubation medium were chromatographed over a 1-mL column of Dowex AG 1-X8 resin, and the fractions were analyzed by scintillation counting. The peak is evident for Ins(1)P in 90-min interferon-treated samples, while Ins(1:2 cycl)P constantly increases along the time of treatment and accumulates to significant concentrations in 24-hour treated samples and in unstimulated cells. Data are the mean of 3 experiments differing ≤5% SD.$

Percentage of radiolabeled inositol phosphates in the water-soluble material (100%). Lymphocytes in the different experimental conditions were incubated in a reaction mixture containing 11.25 μmol/L lyso-PI up to 30 minutes. Once the reaction was stopped, the water-soluble products extracted from the incubation medium were chromatographed over a 1-mL column of Dowex AG 1-X8 resin, and the fractions were analyzed by scintillation counting. The peak is evident for Ins(1)P in 90-min interferon-treated samples, while Ins(1:2 cycl)P constantly increases along the time of treatment and accumulates to significant concentrations in 24-hour treated samples and in unstimulated cells. Data are the mean of 3 experiments differing ≤5% SD.

This Feature Is Available To Subscribers Only