Fig. 3.
![Fig. 3. UV/visible spectrum of F417S recombinant human ferrochelatase. The spectrum of purified recombinant F417S ferrochelatase in elution buffer7 was obtained with a Varian 219 spectrophotometer. Not present are characteristic features resulting from the [2Fe-2S] cluster, which are normally present at 460 nm, 420 nm, and 325 nm in spectra from the wild-type protein.14 A minor absorbance peak at 410 nm is attributed to small amounts of residually bound porphyrin.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/10/10.1182_blood.v91.10.3980/3/blod41005003x.jpeg?Expires=1736058522&Signature=rlVFkDVzes-bbvdpOU2WGxsD1HgNABNz6ALhSFzTMX1Ni85ylKzHoO34ZZ2VG7LIp93mkMtQGip9ouzT6n~zAX5k5TOsRF9rOEkh0KTk4C1MdnXtp~p9E-kGThE3k92BQaDilX83nzpwnwxt8KdOUGQqn-hj65h6uRzpIq5l0gItFPW9~xjZt-MLdUX7xLnE76g61zol2TVu5yhUaW-dJUCeKDKM9AX3qvWBQRY1PIyWgOLDsGA7ZMs58H7w6DwWNTPfvTLaY0Mag2t7xOUUmN61b9fXvqJyiI6v4qSYpgMxSHvOTU49BnaYvhRGlRGxG90Hi2YvPdGC2T7ad5bk9w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
UV/visible spectrum of F417S recombinant human ferrochelatase. The spectrum of purified recombinant F417S ferrochelatase in elution buffer7 was obtained with a Varian 219 spectrophotometer. Not present are characteristic features resulting from the [2Fe-2S] cluster, which are normally present at 460 nm, 420 nm, and 325 nm in spectra from the wild-type protein.14 A minor absorbance peak at 410 nm is attributed to small amounts of residually bound porphyrin.
