Fig. 1.
Inhibitory effect of anti-CD20 MoAb 1F5 on Ramos cells measured by tritiated thymidine incorporation. 104 cells in 100 μL per well were incubated continuously with 0.0001, 0.001, 0.01, 0.1, 1, 10, 100 μg/mL 1F5 in medium containing FBS (A) or medium containing inactivated FBS (B) at 37°C for 24 hours, followed by addition of 0.5 μCi [3H]-thymidine per well and incubating for another 18 hours. Cells incubated in medium without 1F5 were used as control. Data are representative of three concordant experiments. Similar results were obtained with the B1 anti-CD20 antibody (not shown).