Fig. 3.
Effect of fMLP on superoxide production and [3H]-2–DOG uptake by human neutrophils. Neutrophils (106) were incubated with 0.5 μmol/L fMLP for the times indicated (B and D) or for 5 minutes at the concentrations indicated (A and C) and SOD-inhibitable cytochrome c reduction (A and B) and [3H]-2–DOG uptake (C and D) determined. The results are the average of duplicate determinations. For (A) and (B), control absorbances (A550) in the presence of SOD were 0.342 ± 0.003 and 0.305 ± 0.003, respectively, and for (C) and (D), control uptake was 2,613 ± 32 cpm and 2,068 ± 94 cpm, respectively.