Effects of genistein and calphostin C on [³H]-2–DOG uptake by unstimulated and stimulated human neutrophils. Neutrophils (10⁶) were pretreated without (solid bars) or with 100 μmol/L genistein (patterned bars) or 1 μmol/l calphostin C (shaded bars) for 20 minutes before stimulation with 100 ng/mL PMA for 20 minutes or 0.5 μmol/L fMLP for 5 minutes and [³H]-2–DOG uptake determined over 3 minutes in the absence of inhibitor. Results are the average of duplicate determinations.