Phenotypic characterization of HEL cells stained with various anti-adhesion molecule antibodies. HEL cells (105cells each) were incubated with 5 μg of anti-CD29, CD18, CD61, CD49b, CD49c, CD49d, CD49e, CD49f, CD11a, CD11b, CD11c, CD41 or CD44 MoAb in PBS-supplemented 0.5% (wt/vol) BSA and 5 mmol/L EDTA for 30 minutes at 4°C. After incubation, the cells were washed twice with the same buffer and then stained with FITC-labeled anti-mouse IgG sheep F(ab′)2 fragment in the same buffer for 30 minutes. After staining, the cells were washed twice and then analyzed by flow cytometry. For analysis of the expression of adhesion molecules on HEL cells, the specified gate for HEL cells was set in the plot of forward and side scatter. The MoAb used is indicated at the top of each histogram. The shaded peak indicates the expression of the adhesion molecule on HEL cells stained with an anti-adhesion molecule MoAb. The dotted line indicates the background for HEL cells stained with only FITC-labeled anti-mouse IgG F(ab′)2 fragment.
