Expression of the full-length or truncated CD34 and CD34 chimeric receptors on the BaF3 cell transfectants. BaF3 cells were stably transfected with each receptor expression construct as indicated, and individual positive cell clones were selected. (A) Flow cytometry analyses. The vector-transfected BaF3 cells (negative controls, open histograms) or the receptor-transfected BaF3 cells (solid histograms) were stained with the fluorescein isothiocyanate (FITC)-labeled anti-CD34 MoAb HPCA-2. The staining profiles of the BaF3-transfected cells with an isotype-matched control MoAb are the same as those of negative controls (shown as open histograms). (B) Western blot analyses. Cell lysates from the BaF3 cell transfectants were electrophoresed through an 8% SDS-polyacrylamide gel and transferred to a PVDF membrane. The blot was probed with anti-CD34 MoAb QBEND 10 and visualized by ECL detection using goat antimouse IgG conjugated to horseradish peroxidase as a secondary antibody.