BCR/ABL expression and protein tyrosine phosphorylation in individual 32D clone transfectants expressing wild-type or mutant BCR/ABL protein. (A) Expression of wild-type (WT), kinase-deficient mutant (K1172R), or ▵SH3 BCR/ABL proteins was examined in four 32Dcl3 transfectants by Western blotting with anti-ABL MoAb. (B) Phosphorylation of cellular proteins was analyzed after SDS-PAGE of cell lysates from the same clones as in (A) using anti-P.Tyr MoAb 4G10. Results are representative of three independent experiments.